About

Name

Shalina Ousman

University

University of Calgary

Website

people.ucalgary.ca/~sousman/Research.html

Email

[email protected]

Research Keywords

Endogenous protective molecules and mechanisms in Multiple Sclerosis, Peripheral nerve regeneration

Additional Information

Lab Methodologies
  • Experimental allergic encephalomyelitis model
  • Immune cell single and co-culture systems (T cells, neutrophils, macrophages, dendritic cells)
  • Murine ovariectomies and castrations
  • Cell proliferation assays
  • Cytokine measurements using ELISA
  • Immunohistochemistry
  • Western blotting
  • PCR and RT-PCR
  • Peripheral (sciatic) nerve crush and transection
  • Motor nerve conduction velocity measurements
  • Astrocyte cultures

 

Apparatus
  • Level 2 biological cabinets
  • Cell incubators
  • Ultracentrifuge
  • 96 well radioactive cell harvester
  • 96 well radioactive cell counter
  • RT-PCR machine
  • Upright and inverted microscopes
  • ELISA plate reader
  • Motor nerve conduction velocity recorder

 

Roles and Connections

Primary Role

Faculty

CAN Connections

Multiple Sclerosis, Nervous System Injury

Looking For

Accepting graduate students, Accepting postdoctoral fellows

Most Cited Publications

Ousman SS, Kubes P, "Immune surveillance in the central nervous system." Nature neuroscience 15,8 (2012 Jul 26): 1096-101
Acharjee S, Nayani N, Tsutsui M, Hill MN, Ousman SS, Pittman QJ, "Altered cognitive-emotional behavior in early experimental autoimmune encephalitis--cytokine and hormonal correlates." Brain, behavior, and immunity 33, (2013 Oct): 164-72
Palmer AL, Ousman SS, "Astrocytes and Aging." Frontiers in aging neuroscience 10, (2018): 337